What will happen when we reverse an analysis/synthesis system?

1. What happens during the reverse transcription phase?
2. What is the purpose of the reverse transcription reaction?
3. What is the main disadvantage of reverse transcriptase?
4. What does the reverse transcription help us to obtain?

What happens during the reverse transcription phase?

Reverse transcriptase is used to make a cDNA copy of the mRNA. The cDNA sample is then amplified by PCR. This yields multiple copies of cDNA without introns. Reverse transcription followed by PCR allows cloning of genes starting from the messenger RNA, and thus, identifying the expressed exons of the eukaryotic gene.

What is the purpose of the reverse transcription reaction?

Reverse transcription-polymerase chain reaction may be used to look for certain changes in a gene or chromosome or for activation of certain genes, which may help diagnose a disease, such as cancer.

What is the main disadvantage of reverse transcriptase?

The disadvantages of RT-PCR include its complexity and problems associated with its sensitivity, reproducibility, and specificity. Moreover, it suffers from the problems inherent in traditional PCR when it is used as a quantitative method (3).

What does the reverse transcription help us to obtain?

Reverse transcription combined with PCR, or reverse transcription PCR (RT-PCR), allows detection of RNA even at very low levels of gene expression and paves the way for detection of circulating RNA, RNA viruses, and cancerous gene fusions in molecular diagnostics [11-13].