Reverse transcription pdf

1. What is the process of reverse transcription?
2. What is reverse transcriptase PCR PDF?
3. What is the principle of reverse transcriptase?
4. What are the steps in reverse transcriptase PCR?

What is the process of reverse transcription?

In biology, the process in cells by which an enzyme makes a copy of DNA from RNA. The enzyme that makes the DNA copy is called reverse transcriptase and is found in retroviruses, such as the human immunodeficiency virus (HIV). Reverse transcription can also be carried out in the laboratory.

What is reverse transcriptase PCR PDF?

RT-PCR. is reverse of “transcription” hence the term “reverse-transcription.” Reverse-transcriptase is a polymerase enzyme that transcribes. single-stranded RNA into single-stranded DNA called complemen- tary DNA or cDNA using oligo dT primer, gene-specific primers or.

What is the principle of reverse transcriptase?

The principle is to convert RNA into its complementary DNA sequence by reverse transcriptase, to synthesise a second strand with DNA polymerase, and finally to generate a ds cDNA molecule which can be amplified by PCR in the normal way [10].

What are the steps in reverse transcriptase PCR?

Traditionally RT-PCR involves two steps: the RT reaction and PCR amplification. RNA is first reverse transcribed into cDNA using a reverse transcriptase as described here, the resulting cDNA is used as templates for subsequent PCR amplification using primers specific for one or more genes.