Point spread function procedure

1. What is point spread function in microscopy?
2. How do I get PSF from an image?
3. What is PSF engineering?
4. What is PSF in astronomy?

What is point spread function in microscopy?

Introduction. The ideal point spread function (PSF) is the three-dimensional diffraction pattern of light emitted from an infinitely small point source in the specimen and transmitted to the image plane through a high numerical aperture (NA) objective.

How do I get PSF from an image?

To be sure, you can calculate it knowing the optical resolution d = lambda / 2*NA. (NA is the second number written on the objective lens, after the slash after the magnification number.) Then divide d by the pixel spacing in the image and that is the approximate PSF size.

What is PSF engineering?

The PSF is defined as the square of the modulus of the amplitude point spread function, h(v), which has the form(1)h(v)=[2J1(v)v] From: Handbook of Image and Video Processing (Second Edition), 2005.

What is PSF in astronomy?

The Point Spread Function (PSF) is the response of an optical instrument to an impulse. It characterizes how much the observed light distribution has been distorted by the instrument. Therefore knowing the PSF is fundamental in astronomy for morphometric and photometric measurements.