Howtosignalprocessing
- How do you find the point spread function?
- What does point spread function do?
- What is PSF in microscopy?
- How do I get PSF from an image?
How do you find the point spread function?
As a function of the angular pattern size, equation for the normalized PSF intensity can be written as: with a=πDsinα/2, in units of λ, where D is the aperture diameter and α the angular point height in the image plane.
What does point spread function do?
In fluorescence microscopy, the acquired image is always a blurred representation of the actual object under the microscope. This blurring is described by the so-called Point Spread Function (PSF). The PSF describes what a single point in the object looks like in the image.
What is PSF in microscopy?
The ideal point spread function (PSF) is the three-dimensional diffraction pattern of light emitted from an infinitely small point source in the specimen and transmitted to the image plane through a high numerical aperture (NA) objective.
How do I get PSF from an image?
To be sure, you can calculate it knowing the optical resolution d = lambda / 2*NA. (NA is the second number written on the objective lens, after the slash after the magnification number.) Then divide d by the pixel spacing in the image and that is the approximate PSF size.
