Howtosignalprocessing
- How do you read a DNA sequence chromatogram?
- What does a DNA chromatogram tell you?
- How do you analyze Sanger sequencing data?
- What is chromatogram in bioinformatics?
How do you read a DNA sequence chromatogram?
The bases are read in order from left to right and top to bottom (on a chromatogram having more than one row of information). This order corresponds to the 5' end of the sequenced DNA to the 3' end. Such evenly-spaced, clear peaks make base calling straightforward and unambiguous.
What does a DNA chromatogram tell you?
Automated DNA Sequencers generate a four-color chromatogram showing the results of the sequencing run, as well as a computer program's best guess at interpreting that data --- a text file of sequence data.
How do you analyze Sanger sequencing data?
Sanger sequencing analysis is performed on a comparative basis, where the patient's electropherogram is compared against an electropherogram from a DNA sample without a mutation. Any observed differences between the two traces are recorded and analysed for their potential pathogenic effect on the protein.
What is chromatogram in bioinformatics?
A chromatogram (sometimes also called electropherogram) is the visual representation of a DNA sample produced by a sequencing machine (such as Applied Biosystems ABI PRISM 7700 Sequence Detection System).
