A folder of images can be opened as a stack either by dragging and dropping the folder onto the 'ImageJ' window or or by choosing File▷Import▷Image Sequence… ↓ To create a new stack, simply choose File▷New▷Image… [n]↓ and set the Slices field to a value greater than one.
How do I combine stacks in ImageJ?
Combining stacks
Just open each image stack separately, then run Image > Stack > Merge Channels..., assigning each image stack to the desired channel. The result will be a composite of each respective plane.
How do I stack images?
To stack photos, highlight the photos you want to group and go to the Edit drop-down menu. Select Edit > Stacks > Group into Stacks (Ctrl or ⌘G). You can also right-click on the selected images and select 'Group into Stacks'. The stack number is in the upper right-hand corner of the top image.
How do I save a stack in ImageJ?
At any point in time you can save the image stack you are working on by selecting File > Save as > Image Sequence… from the menu bar 2.