Calibration

How to make a calibration curve

How to make a calibration curve
  1. What should be included in a calibration curve?
  2. How many solutions are needed for a calibration curve?

What should be included in a calibration curve?

A calibration curve is a method used in analytical chemistry to determine the concentration of an unknown sample solution. It is a graph generated by experimental means, with the concentration of solution plotted on the x-axis and the observable variable — for example, the solution's absorbance — plotted on the y-axis.

How many solutions are needed for a calibration curve?

For an initial assessment of the calibration function, as part of method validation for example, standards with at least seven different concentrations (including a blank) should be prepared.

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