Short answer: yes, there will be a small difference in the size of files.
Does audio increase file size?
Audio. Sometimes audio can create larger file sizes, though most of the time recording devices and software will compress your audio so as not to create large file sizes. In addition, it is very rare that uncompressed audio is going to make an audible difference to your viewers.
What determines the size of a sound file?
To determine the file size of an audio file, we have to multiply the bit rate of the audio by its duration in seconds. As a result, we get file size values in terms of kilobits and megabits.
Why is my MP3 file so big?
If your file was recorded at a higher bitrate (i.e., 192kbps or 256kbps) the file size will be comparatively large. You can reduce the size of such an MP3 file by Importing it into your audio recording software and then Exporting it again using the recommended bitrate (128kbps) and sample rate (44.1 kHz).