Howtosignalprocessing
- What is confocal microscopy used for?
- What is the difference between confocal and fluorescence microscopy?
- What is a confocal microscope and what are its advantages?
- What are the examples of confocal microscope?
What is confocal microscopy used for?
Confocal microscopy is widely used for fluorescence imaging in the life sciences. The last decade has seen advances in illumination sources, detectors, fluorescent probes, optics, and sample preparation techniques, which provide improvements in different combinations of speed, depth, and resolution.
What is the difference between confocal and fluorescence microscopy?
The fluorescence microscope allows to detect the presence and localization of fluorescent molecules in the sample. The confocal microscope is a specific fluorescent microscope that allows obtaining 3D images of the sample with good resolution.
What is a confocal microscope and what are its advantages?
Confocal microscopy technologies
Confocal laser scanning and spinning-disk confocal microscopy allow researchers to generate 3D images of organelles within living cells and examine changes that occur in cells over time. Confocal laser scanning microscopes only allow for a relatively slow image acquisition speed3.
What are the examples of confocal microscope?
There are three types of confocal microscopes: laser scanning microscopes, which use a sharply focused laser that scans over the sample; spinning disk confocal microscopes, which use a disk with pinholes cut into it that are arranged in the shape of a spiral; and programmable array microscopes (PAM), which work much ...
