Deconvolution of sidelobes in a point spread function?

1. What affects point spread function?
2. What does point spread function do?
3. What is point spread function in microscopy?
4. What is convolution and deconvolution?

What affects point spread function?

So What Affects the Point Spread Function? The PSF varies depending on the wavelength of the light you are viewing: shorter wavelengths of light (such as blue light, 450 nm) result in a smaller PSF, while longer wavelengths (such as red light, 650 nm) result in a larger PSF and, therefore, worse resolution.

What does point spread function do?

In fluorescence microscopy, the acquired image is always a blurred representation of the actual object under the microscope. This blurring is described by the so-called Point Spread Function (PSF). The PSF describes what a single point in the object looks like in the image.

What is point spread function in microscopy?

Introduction. The ideal point spread function (PSF) is the three-dimensional diffraction pattern of light emitted from an infinitely small point source in the specimen and transmitted to the image plane through a high numerical aperture (NA) objective.

What is convolution and deconvolution?

In mathematics, deconvolution is the operation inverse to convolution. Both operations are used in signal processing and image processing. For example, it may be possible to recover the original signal after a filter (convolution) by using a deconvolution method with a certain degree of accuracy.